Ewing's sarcoma.

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Abstract

[Indexed for MEDLINE] 3. Methods Mol Biol. 2022;2373:283-296. doi: 10.1007/978-1-0716-1693-2_17. 3D Biofabricated In Vitro Models of Vascularized and Mineralized Bone Tissues. Colombo MV(1)(2), Bersini S(1), Arrigoni C(1), Moretti M(3)(4)(5). Author information: (1)Regenerative Medicine Technologies Laboratory, Ente Ospedaliero Cantonale, Lugano, Switzerland. (2)Department of Chemistry, Materials and Chemical Engineering (CMIC) "Giulio Natta", Politecnico di Milano, Milan, Italy. (3)Regenerative Medicine Technologies Laboratory, Ente Ospedaliero Cantonale, Lugano, Switzerland. matteo.moretti@eoc.ch. (4)IRCCS Istituto Ortopedico Galeazzi, Milan, Italy. matteo.moretti@eoc.ch. (5)Facoltà di Scienze Biomediche, Università della Svizzera Italiana, Lugano, Switzerland. matteo.moretti@eoc.ch. This protocol describes a comprehensive practical guide for the biofabrication of 3D in vitro models of vascularized and mineralized bone Minitissues. These models give the possibility to study the contribution of physical and biochemical parameters on bone vascularization, as well as the osteoblast/osteoclast mediated matrix remodeling. Based on the specific pathophysiological processes to be investigated, the 3D bone Minitissues allow to select the most suitable cell composition, by coculturing up to four cell types, and to customize the material properties of the hydrogel matrix. Considering their versatility, these 3D bone Minitissues could be relevant for the recapitulation of bone pathologies such as bone tumors and metastases and could be and used as screening platforms to test antimetastatic drugs. © 2022. Springer Science+Business Media, LLC, part of Springer Nature. DOI: 10.1007/978-1-0716-1693-2_17