Injectable Peptide Therapy: A Primer for Orthopaedic and Sports Medicine Physicians.

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Abstract

[Indexed for MEDLINE] Conflict of interest statement: One or more of the authors has declared the following potential conflict of interest or source of funding: J.N.L. has received speaking fees from Stryker and hospitality payments from Innocoll Biotherapeutics. G.R.H. has received consulting fees from Arthrex. S.C.G. has received speaking fees from Arthrex; has received consulting fees from Biomet, Zimmer, and Exactech; has received royalties from Zimmer; and holds stock or stock options in Kelvi. AOSSM checks author disclosures against the Open Payments Database (OPD). AOSSM has not conducted an independent investigation on the OPD and disclaims any liability or responsibility relating thereto. 7. Arthritis Res Ther. 2022 Jun 28;24(1):158. doi: 10.1186/s13075-022-02832-8. Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats. Song X(1), Zhao M(1), Tang J(1), Ma T(1), Bai H(1), Wang X(1), Liu L(1), Li T(1), Xu X(1), Sheng X(1), Zhao B(1), Wang Y(1), Wang T(1), Guo Y(1), Zhang X(1), Gao L(2). Author information: (1)Heilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agriculture University, 600, Changjiang Road, Harbin, 150030, China. (2)Heilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agriculture University, 600, Changjiang Road, Harbin, 150030, China. gaoli43450@163.com. BACKGROUND: Light alteration affects the internal environment and metabolic homeostasis of the body through circadian rhythm disorders (CRD). CRD is one of the factors that induce and accelerate osteoarthritis (OA). Therefore, the aim of this study was to evaluate the effects of continuous dark-light (DL) cycle on joint inflammation, bone structure, and metabolism in normal and OA Sprague-Dawley (SD) rats. METHODS: Interleukin (IL)-1β, IL-6, inducible nitric oxide synthase (iNOS), and tumor necrosis factor (TNF)-α were used to evaluate the systemic inflammation in rats. The pathological changes and inflammatory reactions of the cartilage and synovium of the knee joint in rats were evaluated by Safranin O-fast green and immunological staining. Bone turnover was assessed by histomorphometry and μCT scanning, as well as bone metabolism markers and proteins. The expression changes of clock proteins BMAL1, NR1D1, PER3, and CRY1 in representative tissues were detected by western blotting. RESULTS: DL cycle significantly inhibited body weight gain in normal and OA rats. The levels of proinflammatory factors in the peripheral blood circulation and degradation enzymes in the cartilage were significantly decreased in OA+DL rats. DL cycle significantly destroyed the structure of subchondral bone in hindlimbs of OA rats and reduced trabecular bone numbers. The decrease of bone mineral density (BMD), percent bone volume with respect to total bone volume (BV/TV), trabecular number (TB.N), osteoclast number, and mineralization could also be found. The ratio of the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin (RANKL/OPG) in the bone marrow of OA rats was markedly increased under DL, along with the activation of the mononuclear/phagocyte system. The expression of representative clock proteins and genes BMAL1, PER3, and CRY1 were markedly changed in the tissues of OA+DL rats. CONCLUSIONS: These results suggested that DL cycle dampened the arthritis and promoted bone resorption and bone mass loss. DL cycle affects bone turnover by regulating osteoclast production in osteoarthritic rats. © 2022. The Author(s). DOI: 10.1186/s13075-022-02832-8 PMCID: PMC9238010